Phytochemical analysis of Curculigo orchioides and its cytotoxic effect on lung adenocarcinoma cancer cell line (NCI-H522) Aloysius Khyahrii S, Sharanya K, Kini Sudarshan, Milan Gaurav R, Hegde Smitha* Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Deralakatte, Mangalore-575018, Karnataka, India *Corresponding author e-mail: smitha.hegde@nitte.edu.in
Online published on 15 October, 2020. Abstract Curculigo orchioides commonly called as ‘Kali Musli’ is an endangered medicinal plant, extensively used in Indian and Chinese indigenous medicines. The plant has several medicinal properties like anti-inflammatory, anti-diabetic, anti-cancer etc. The present study aims to examine the cytotoxic activity of aqueous and methanolic extract of roots and leaves of C. orchioides on human lung adenocarcinoma NCI-H-522 cancer cell line. In-vitro cytotoxicity was determined by MTT assay. The tests were conducted using various doses of the plant extracts. The crude extracts did not show IC50 even at 500 μg/ml when tested on NCI-H-522 cancer cell line in vitro. Our present finding indicate that aqueous fresh root extracts and methanolic dried root extracts are significantly toxic as compared to leaf extracts. Cytotoxic property of the plant root is attributed to the presence of alkaloids and phenols. Top Keywords Curculigo orchioides, MTT assay, NCI-H-522 cell line, Phenolic glycoside, Secondary metabolites. Top |