Role of vaccine virus in interfering the pathogenesis of wild strain of duck plague virus (DPV) Pathak Mamta4, Begum S. Ara1,4, Arif S.A.2,4, Konwar Nayanmoni3,4, Patel S.K.2, Barman N. Nath3,4,*, Rahman Taibur1,4 4Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Uttar Pradesh, India; 1Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara-781022, Guwahati, India. 2Department of Veterinary Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara-781022, Guwahati, India. 3Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara-781022, Guwahati, India. *Address for Correspondence Dr. Nagendra Nath Barman, Department of Veterinary Microbiology, College of Veterinary Science, Khanapara, Guwahati-781022, Assam, e-mail: nnbarman@gmail.com
Abstract Duck plague or duck viral enteritis is an acute highly contagious disease of duck, geese and swan of all ages caused by Anatid Herpesvirus-1 and characterized by high morbidity and mortality and sudden death. In the present study, revival, pathogenesis and pathology of wild strain (DP/As-Km/0019) of duck plague virus (DPV) were studied in 2 month old ducklings. In order to study the role of vaccine virus in interfering the pathogenesis of wild strain of DPV in ducklings, a total of 24 numbers of ducklings were divided into four groups. Group I was vaccinated followed by challenge after 24 hours, Group II was challenged 48 hours prior to vaccination, Group III was kept as positive control, whereas Group IV was kept as negative control without vaccination or challenge. Clinical signs in inoculated ducklings were weakness, lethargy, depression, loss of appetite, ruffled feathers, nasal discharge, lacri- mation, pasted eyelids with periorbital ring formation and greenish diarrhoea. On necropsy, gross lesions were observed in brain, oesophagus, liver, spleen, heart and intestine. Microscopically, vascular and degenerative changes like congestion, haemorrhages, necrosis and formation of diphtheritic membrane were recorded, whereas fatty changes, emphysema and coagulative necrosis were seen in all parenchymatous organs. Presence of viral DNA was detected in clinical samples as well as in tissue samples by molecular assay and confirmation of duck plague viral antigen in tissue samples was done by sandwich ELISA. In conclusion, vaccination could fully protect the ducks in endemic areas, however, near protection could be found after 48 hours of challenge. How to cite this article: Pathak, M., Begum, S. Ara, Arif, S.A., Konwar, N., Patel, S.K., Barman, N. Nath and Rahman, T. Role of vaccine virus in interfering the pathogenesis of wild strain of duck plague virus (DPV). (2019). Indian J. Vet. Pathol., 43(3): 202–210. Top Keywords Assam, Duck plague virus, Pathogenesis, Vaccine. Top |