Year : 2017, Volume : 41, Issue : 3
First page : ( 210) Last page : ( 213)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2017 September 1.
Article DOI : 10.5958/0973-970X.2017.00050.5

Comparative diagnosis of canine mammary tumours by cytology and histopathology

Acharya A.T.¹, Ghodasara D.J.¹, Joshi B.P.¹, Dave C.J.¹, Prajapati K.S.¹, Jani Purnima B.¹, Khorajiya J.H.^1,*

¹Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, AAU, Anand-388001, Gujarat, India

*Corresponding author: e-mail: jainudeen_1990@rediffmail.com

Received:  26  October,  2016; Accepted:  3  June,  2017.

Abstract

The aim of the present study was to correlate cytological diagnosis after pre-operative fine needle aspiration biopsies from canine mammary tumours with that of histopathological findings. In prospective study, 40 fine-needle aspirate samples were obtained from 40 different cases, out of that, 15 cases were diagnosed mammary gland tumours. Histopathology results included four cystic papillary adenoma, three benign mixed mammary tumours, two each of papillary adenocarcinoma, solid adenocarcinoma, and mammary gland adenoma, and one each of benign mammary myoepithelioma and malignant myoepithelioma. The neoplastic cells in smears were round to caudate, exhibiting anisocytosis, spherical to oval nuclei, anisokaryosis, abnormal nuclear/nucleolar shapes, anisonucleoli, abnormal chromatin pattern, multinucleation and mitotic figures. In case of benign category cells were uniform and in large clusters, having clear cytoplasmic boundaries. On comparing results of cytological diagnosis with the histopathology revealed that 86.66% (13/15) cases of benign and malignant tumours had positive correlation.

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Keywords

FNAC, Cytology, Canine, Histopathology, Mammary tumour.

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Figures

Fig.1.: Papillary adenocarcinoma showing the predominance of pleomorphic glandular epithelial cell cluster, anisokaryosis, anisocytosis and double nucleus (arrow). H&E ×480; TopBack

Fig.2.: Solid adenocarcinoma showing abnormal chromatin (short arrow), mitotic figures (chevron) and anisonucleosis (long arrow). H&E ×480; TopBack

Fig.3.: Section of papillary adenocarcinoma showing papillary out- growths of epithelium, projecting in to the lumen of acini with a connective tissue stalk of variable lengths and thickness over lined by multiple layers of proliferating epithelial cells. H&E ×120; TopBack

Fig.4.: Section of benign mixed mammary tumour showing proliferation of epithelial and connective tissue components, with presence of cartilaginous and osseous tissues. H&E ×48. TopBack

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