Codon Usage Variation in Moderately Thermophilic Kyrpidia tusciae (Bacillus tusciae) Shende Kishor*, Mishra Anita**, Gothalwal Ragini*** Department of Biotechnology and Bioinformatics Center, Barkatullah University, Bhopal-462026, M.P., India *E-mail: kishor556@hotmail.com
**anitamishra555@hotmail.com
***ragini_gothalwal@yahoo.com
Online published on 31 October, 2013. Abstract Bacillus tusciae Bonjour & Aragno 1994, is moderately thermoacidophilic, chemolithotrophic, hydrogen-oxidizing, facultative in solfataras, spore former, non-metaboliser of sugar. Reduced genome size with reduced number of G/C ending codon specific tRNA is adaptability to Kyrpidia tusciae. Correspondence analysis has revealed variation in codon usage pattern of translation, carbohydrate transport and metabolism, nucleotide transport and metabolism, cell cycle control, cell motility and secondary metabolites production. Kyrpidia tusciae is slow growing bacteria and lacking the efficiency in metabolizing carbohydrates, may be due to changed codon usage pattern influencing the gene expression rate. Moderately hemophilic nature can be correlated with the GC richness of the genome and dominance of G/C ending codons. Strand wise replication selection is one of the factors influencing codon usage bias was observed in plus strand. Nc-GC3 plot has indicated translational selection as principle factor in shaping codon usage and mutational bias as secondary one. Genes of the processes, intracellular trafficking, defense mechanism, cell motility and secondary metabolites production are composed of varying codon usage pattern may be due to influence of horizontal gene transfer or high level of mutations. Top Keywords Bacillus tusciae, Kyrpidia tusciae, Codon Usage, Mutational Bias, Transaltional Selection. Top |