Simultaneous determination of five bioactive constituents in fruit rind extracts of Garcinia lanceifolia using a validated UHPLC-PDA method and evaluation of antioxidant activity Kureshi Azazahemad A.12, Patel Jinal2, Dholakiya Chirag3, Kar Ashish4, Mirgal Amit5, Salvi S.P.6, Hussain Tabaruk7, Kumari Premlata1, Tripathi Sharad K.2, Singh Raghuraj2, Gada Urja2, Kumar Satyanshu2,* 1Department of Applied Chemistry, SVNIT, Surat-395007, Gujarat, India 2ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand-387310, Gujarat, India 3Waters (India) Pvt. Ltd., S.G. Highway, Ahmedabad-380015, Gujarat, India 4The Energy Research Institute-NE, Guwahati, Assam, India 5Gogate Joglekar College, Ratnagiri-415612, Maharashtra, India 6Regional Fruit Research Station, Vengurla-416516, Maharashtra, India 7Sarat Chandra Singha College of Agriculture, Assam Agricultural University, Dhubri, Assam, India *Corresponding author e-mail: satyanshu66@gmail.com
Online published on 2 April, 2021. Abstract Xanthones and polyisoprenylated benzophenones found in Garcinia species are groups of secondary metabolites with a wide range of bioactivities. Three xanthones namely α-mangostin, β-mangostin, γ-mangostin and two polyisoprenylated benzophenone namely xanthochymol and isoxanthochymol were identified and quantified in the extracts prepared from fruit rinds of G. lanceifolia using a validated UHPLC-PDA method. Total phenolic content of extracts prepared from G. lanceifolia fruit rinds with solvents of varying polarity was determined by the Folin-Ciocalteu method. These extracts were also evaluated for their antioxidant potential using three different in vitro assays namely DPPH free radical, ABTS cation radical and reducing power. α-mangostin was not quantified in hexane and chloroform extracts. β-mangostin could not get quantified in hexane extract. γ-mangostin was not detected in any of the six extracts. The maximum content of xanthochymol (289.26 ± 0.31 mg/g) was detected in hexane extract. Isoxanthochymol content (9.91 ± 0.01 mg/g) was maximum in hexane extract and it was followed by chloroform extract (2.67 ± 0.01 mg/g). The highest total phenolic content was recorded for hexane extract (18.60 ± 1.95 mg/g GAE). In vitro assays established that G. lanceifolia extracts exhibited significant antioxidant activity. Top Keywords Xanthones, Polyisoprenylated benzophenones, Total phenolic content, Antioxidant activity. Top |