The Journal of Indian Botanical Society
Year : 2021, Volume : 101, Issue : 3
First page : ( 224) Last page : ( 231)
Print ISSN : 0019-4468. Online ISSN : 2455-7218.
Article DOI : 10.5958/2455-7218.2021.00028.0

The effects of (Z)-7-Methoxy-1, 5-Dihydrobenzo [C] Oxepine on In vitro culture of curcuma longa L

Ghosh Arghya¹*

¹U.G. & P. G. Department of Botany, Darjeeling Government College, Darjeeling

*Email: agbotphd@rediffmail.com

Online published on 19 October, 2021.

Abstract

The plant Curcuma longa L. is a pharmaceutically important one and almost all the parts of the plant especially rhizomes were reported to contain different types of terpenoid. Rhizomatous explants from three months old buds were cultured on Murashige and Skoog’s (MS) medium supplemented with different concentrations of cytokinins and auxins. When the culture was performed in MS medium supplemented with 3 mg/L of 6-benzylamino purine, it yields 7.12±0.34^bc numbers of shoots with an average shoot length of 8.32±0.47^a“ cm. The elongated shoots produced 6.12±0.73^def roots with an average length of 4.93±0.30^abc on MS medium supplemented with 2.5 mg/L of Indole 3 butyric acid (IBA) and showed 82% survivability after hardening. When the culture on 3 mg/L of 6-benzylamino purine was supplemented with 12 mg/L of the (Z)-7-methoxy-1, 5-dihydrobenzo [c] oxepine isolated from Curcuma caesia Roxb., multiple numbers of shoots having an average shoot length of 3.29±0.17^c cm were produced. The elongated shoots produced roots with an average length of 7.91±0.93^1Jk on MS medium supplemented with 2.5 mg/L of Indole 3 butyric acid (IBA) and 14 mg/L of (Z)-7-methoxy-1,5-dihydrobenzo [c] oxepine. Hence, it can be concluded that although this terpenoid is a shoot growth retardant yet acts as elicitor for rooting.

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Keywords

Curcuma longa L., Curcuma caesia Roxb., (Z)-7-methoxy-1, 5-dihydrobenzo [c] oxepine (MDHBO), Plant growth regulator, 6-Benzylamino purine, Indole 3 butyric acid..

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