Indirect organogenesis and regeneration of Macrotyloma uniflorum (Lam) Verdc Palanisamy Karthika2, Krishnan Vasanth2*, Gurusamy Dhandapani1 1Department of Botany, Kongunadu Arts and Science College (Autonomous), Bharathiar University, Coimbatore641 029, Tamil Nadu, India. 2Molecular Biology Lab, Department of Botany, Bharathiar University, Coimbatore641 046, Tamil Nadu, India. E-mail: vasanthlabbu@gmail.com
Online published on 19 October, 2021. Abstract The present research was performed to develop an indirect organogenesis protocol in Macrotyloma uniflorum via callus by using cotyledon and epicotyledon explants. Cotyledon explants were produce the best result on MS medium supplemented with 3.0 mg/L BAP. The maximum percentage (97%) of regeneration was obtained with the combination of BAP+GA3 (2.0+1.0 mg/L) from cotyledon explants. Well established root system was developed from regenerated shoot lets in the medium combination with BAP+NAA (1.0+1.0 mg/L). Cotyledon explants were showed more responsible than epicotyledon in terms of callus induction and plant regeneration. This in vitro, developed plant lets were successfully acclimatized to the pots and established in the green house. This standardized in vitro regeneration system will facilitate further to development of a reliable procedure to accomplish genetic manipulation in this genus. Top Keywords Cotyledon, Callus induction, Epicotyledon, Indirect organogenesis, Regeneration. Top |