Pichia pastoris-A Model System for Expression of Recombinant Proteins Ningaraju T. M., Sangh Madhusudhan R. Chandramohan*, Gowda P. H. Ramanjini Department of Plant Biotechnology, UAS, GKVK, Bangalore-65 *E-Mail id: rsmadhu@gmail.com
Online published on 31 October, 2013. Abstract The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant proteins. Pichia pastoris has become a highly popular expression host for the production of a wide variety of intracellular and extracellular recombinant proteins of interest. Recent advances in the development of yeast as a host for the production of heterologous proteins have provided a catalogue of new applications, methods and system components, which will help us to understand about more details and applications of the system even further. Pichia is most favoured because of the simplicity of techniques needed for the molecular genetic manipulation; the ability to produce foreign proteins at high level, the capability of performing many eukaryotic post-translational modifications (glycosylation, disulfide bond formation and proteolytic processing) and finally the availability of the expression system as a commercially available kit. In this review we discussed about factors influencing folding of proteins, different host system for expressing proteins, methanol metabolism, expression vectors, AOX promoters, selectable markers, host strains, Strategies to increase the protein production and strategies to control proteolysis. Top Keywords Pichia Pestoris, Protein production, probedying. Top |