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Enhancement in Biopotentiality of Trichoderma viride (Pers.) through UV Mutagenesis against Rhizoctonia solani Saini K.K.*, Nathawat B.D.S. Division of Plant Pathology, Rajasthan Agricultural Research Institute, Durgapura, (Jaipur), Rajasthan-302 018 *email: kamalsainipathology@gmail.com
Online published on 2 January, 2017. Abstract Four efficient mutant strains were obtained from the wild T.viride strain after UV treatment of different time period exposure ranging from20 to80 minutes. Selected mutants were tested for in vitro inhibition of Predominant soilborne fungal pathogen Rhizoctonia solani by using various plate culture techniques (dual culture, inverted plate technique) for their bio-efficacy. In dual culture studies inoculation with T. viride mutant TvM2-UV2/60 showed 80.37 per cent mycelial inhibition of R. solani which was maximum over other mutants and wild strain under study. Studies on volatile compounds that released by T. viride mutant TvM2UV2/60 inhibited mycelia growth rate of R. solani 82.12 per cent over other mutants and wild strain under study in vitro. Mutants showed better sporulation and highest growth on PDA plates and covered whole plate in 4 days than their wild type strain in 7 days. In pot culture experiment, T. viride mutants enhanced root growth than untreated control and thus helped to enhance growth of tomato seedlings. Top Keywords Trichoderma viride, UV Mutagenesis, Rhizoctonia solani. Top | |
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