In Vitro Mass Propagation of Scoparia dulcis L. Through Nodal Segments Karki Krishna Bahadur1,*, Paudel Sudip, Joshi Sanu Devi1 1Central Department of Botany, Tribhuvan University, Kritipur, Kathmandu, Nepal *E-mail: kkarki2000@gmail.com
Online published on 23 May, 2013. Abstract Scopari ducis L., one of the highly medicinal plant found in tropical andsubtropicalregion.Locallyitis calledas “chinijhar” or “mithajhar”. An effective protocol was established for mass propagaton of Scoparia dulcis (Family: Scrophulariaceae). The main phytochemicals studied are scopadulcic acids A and B, scopadiol, scopadulciol, scopadulin, scoparic acids A, B, and C, and betulinic acid. An antidiabetic compound, ‘amellin’ has been reported in leaf and stem of the fresh green plant. Nodal explants were used for the in vitro mass propagation of this plant and the best result for regeneration of shoot was found 8.25 in MS medium supplemented with 1mg/l BAP and so, BAP is the best phytohormone for the regeneration of shoot than the combination with auxin. Similarly, the best result for induction of roots from shoots were found 7.25 in ½ concentration of MS medium supplemented with 0.5mg/l IBA and 12.50 in MS medium supplemented with 0.75mg/l IBA. So, IBA alone is the best phytohormone for the regeneration of shoots then combination with NAA. 75% rooted plantlets was successfully transferred and survived in natural environment. Top Keywords Scopariadulcis, BAP, NAA, IBA, amellin, in vitro. Top |