Development and Validation of RP-HPLC Method for Determination of Febuxostat in Bulk and Pharmaceutical Dosage Formulations Kamble Pragati R.1,*, Pandagale Nikita V.1,**, Ghatage Omkar D.1,***, Patil Pramod B.2, Patil S. V.3 1Department of Pharmaceutical Quality Assurance, Ashokrao Mane College of Pharmacy, Pethvadgaon, Kolhapur - 416112, Maharashtra, India 2Department of Pharmaceutical Chemistry, Ashokrao Mane College of Pharmacy, Pethvadgaon, Kolhapur - 416112, Maharashtra, India 3Department of Pharmaceutics, Ashokrao Mane College of Pharmacy, Pethvadgaon, Kolhapur - 416112, Maharashtra, India *Corresponding Author E-mail: pragatikamble7001@gmail.com
**nikitavpandagale0297@gmail.com
***ghatageomkar4041@gmail.com
Online Published on 03 October, 2023. Abstract A new accurate, specific, precise, high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of Febuxostat in bulk and in its pharmaceutical dosage forms of tablet. Acetonitrile: Methanol (70:30) was used as the mobile phase at a flow rate of 1.0ml/min using a Symmetry C18 column Finepack SIL C18T-5 (250x4.6mm, 5m). The effluent was spectroscopically monitored at 314nm. The intraday and inter-day precisions showed coefficients of variation ranged from 0.62% to 2.78% at different levels of concentrations. The calibration curve was linear with a correlation coefficient of 0.9947. The averages of the absolute and relative recoveries were found to be 98.67% to 98.96%. The proposed HPLC method was successfully applied to quantify the amount of Febuxostat in bulk and dosage forms in quality control. Top Keywords Febuxostat, HPLC, RP-HPLC, Regression, Spectroscopically. Top |