Development of UV- spectrophotometric method for simultaneous estimation of metformin and piperine in combined tablet dosage form Venkatesh Sama, Laxmi Vijaya, Chiluka Rajeswari*, Padmavathi Yenumula Pillar No 23, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad *Corresponding Author E-mail: rajeswari930@gmail.com
Online published on 4 March, 2023. Abstract Piperine is an alkaloid obtained from >Piper nigrum> Linn., is known to modify the biotransformation of the drugs by inhibiting Cytochrome P450 forms. Piperine inhibits the bio-transformation and metabolism of metformin leading to higher levels of drug in systemic circulation, thus increases the bioavailabilty when co-administered with metformin. In the present study a simple, accurate, precise, economical procedures, entailing neither irksome sample treatment nor tedious extraction process a combined tablet dosage form of metformin and piperine have been developed for the simultaneous estimation of piperine and metformin using UV- Spectrophotometry method. The developed method was based on employing simultaneous equation method for analysis of both the drugs. Metformin and piperine have shown absorbance maxima at 237nm and 342nm respectively, in methanol and the linearity was obeyed over the concentration range of 2-10μg/ml and 2-10 μg/ml respectively for metformin and piperine. The validation parameters like precision, accuracy, limit of quantitation (LOQ), limit of detection (LOD), were determined according to ICH guidelines. All the results were found to be within the limits. Top Keywords Piperine, Metformin, Biotransformation, UV- Spectrophotometry, Simultaneous equation method. Top |