Effect of caffeine on frog muscle Kumar Sanjay1,*, Tomar Prakash1, Rao J. Prakasha2 1Assistant Professor, Dept. of Physiology, Sikkim Manipal Institute of Medical Sciences, SMU, Tadong, Gangtok, Sikkim, 737102 2Professor, Dept. of Physiology, KMU, Manipal University *Corresponding Author: E-mail: drsan74@gmail.com
Online published on 18 December, 2015. Abstract Aim The aim of the present investigation is to study the effects of caffeine on frog skeletal, cardiac and smooth muscle tissues. Material and Methods In this study muscle strips were obtained from Sartorius muscle, heart muscle and rectum of frog after pithing the animal. Caffeine (30mM) was administered in aqueous form with the help of Micropipette directly upon the isolated muscle sample. These isolated samples were prepared, fixed and analyzed with the help of laser beam where linear movement of muscle contraction is converted to angular movement which was recorded on the graph paper and further statistical analysis was done using student's t-test to determine significance of differences between means. A “p” value of < 0.05 was considered significant. Conclusion Caffeine (30mM) induces sustained contraction in skeletal and smooth muscle, but cardiac muscle does not respond to caffeine though it increases the natural beat of sinoatrial preparation. Top Keywords Caffeine, laser beam, student's t-test, pithing. Top |