Effect of temperature, pH, and media on growth and sporulation of five isolates of Dactylaria brochopaga Kumar Niranjana,1, Singh K. P.b aDepartment of Crop Protection, Faculty of Agriculture, Ibrahim Badamasi Babangida University, Lapai, Niger State, Nigeria e-mail: niranjanibbu@gmail.com bDepartment of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India e-mail: kpsmppbhu@redifrmail.com 1Corresponding author
Online published on 14 October, 2011. Abstract The growth of all the five isolates of D. brochopaga was maximum at 30°C, whereas, no any growth was observed at 10°C and mycelial dry weight of the isolates was maximum at pH 7 followed by pH 6 and 8. Among the six synthetic/natural media, Czapek's agar medium supported maximum growth of all isolates of D. brochopaga followed by rabbit dung extract medium, whereas, Richard's medium supported minimum. Among the five isolates of D. brochopaga, isolate D recorded maximum radial growth on all the media tested. Among the bran media, lentil bran medium showed maximum growth of all the isolates of the fungus except isolate D which recorded significantly higher growth on pigeon-pea bran medium. Minimum radial growth of all isolates was recorded on pea bran medium. The best sporulation of isolates D was recorded on wheat bran medium followed by pigeon pea medium. Of the five grain agar media tested, sorghum grain medium supported best growth, whereas, bajra grain medium recorded minimum growth of all the isolates. The best sporulation of all the isolates was recorded on sorghum grain medium closely followed by barley grain medium. Mass culture of isolate D of D. brochopaga showed best growth in 25 days at 25°C, on sorghum grain particularly when amended with CaCO3 and CaSO4. Top Keywords Dactylaria brochopaga, brans, grains, nematode-trapping fungi, mass culture. Top |